Protective effect of peroxisome proliferator-activated receptor ? on TNF?-induced injury of inner medullary collecting duct cells / 第二军医大学学报

ABSTRACT

Objective:To observe the protective effect of peroxisome proliferator-activated receptor ?(PPAR?) on TNF?-induced injury of the inner medullary collecting duct(IMCD) cells.Methods: Cultured IMCD cells were randomly divided into blank control,TNF? stimulation,PPAR? transfection(pPPAR?-control) and PPAR? transfection + TNF? stimulation groups.Stimulation was induced with 50 ng/ml TNF? for 24 h and mouse wild-type PPAR? plasmid was used to transfect IMCD cells.Cell supernatant MCP-1 or TGF?_1 was detected by ELISA method.Results: IMCD cells transfected with wild-type PPAR? plasmid had high expression of PPAR? mRNA and protein.The contents of MCP-1 and TGF?_1 in the supernatant were similar in blank control group and pPPAR?-control group.Compared with blank control group,TNF? stimulation group had decreased contents of MCP-1 and TGF?_1in the supernatant(P