Influence of sodium selenite on expression of p38 mitogen-activated protein kinase and peroxisome proliferator-activated receptor ? in rat mesangial cells line HBZY-1 / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12)1985.
Article
in Chinese
| WPRIM
| ID: wpr-559230
ABSTRACT
Objective:
To observe the influences of sodium selenite on expression of p38 mitogen-activated protein kinase(p38MAPK) and peroxisome proliferator-activatee receptor ?(PPAR?) in rat mesangial cells line HBZY-1,so as to study the role of p38MAPK and PPAR? in diabetic nephropathy and the mechanism by which sodium selenite prevents diabetic nephropathy.Methods:
Rat mesangial cell line HBZY-1 was incubated with high glucose,high insulin,H_(2)O_(2) and advanced glycosylation end products(AGEs) separately before and after HBZY-1 cells were pre-treated with SB203580(p38MAPK special inhibitor)or sodium selenite.Cells receiving no stimulation were taken as control.The expression of p38MAPK protein and PPAR? mRNA was detected respectively by immunohistochemistry assay and RT-PCR in all groups and the results were compared.Results:
High glucose,high insulin,H_(2)O_(2) and AGEs all activated p38MAPK,increased phospho-p38MAPK expression and decreased the expression of PPAR? mRNA in rat mesangial cells line HBZY-1.The expressions of phospho-p38MAPK protein was markedly inhibited by sodium selenite,while the expression of PPAR? mRNA was significantly increased by SB203580 or sodium selenite in rat mesangial cells lines HBZY-1(P
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Academic Journal of Second Military Medical University
Year:
1985
Type:
Article
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