EFFECTS OF SELENIUM ON GENE EXPRESSION OF PHOSPHOLIPASE D GENE IN LIVER IN DIABETIC RATS / 营养学报
Acta Nutrimenta Sinica
;
(6)1956.
Article
in Chinese
| WPRIM
| ID: wpr-559307
ABSTRACT
Objective:
To study the regulation of selenium on gene expression of phospholipase D (PLD) gene in relation to liver metabolism of diabetic (DM) rats.Method:
Differential display cDNA fragments were isolated from former research project in diabetic rats which were supplemented orally with 50 g/kg bw d selenium for 60d, and were cloned, sequenced, and the homology was analyzed. RT-PCR was made using the primers designed according to the sequences of cDNA fragments.Results:
In differential display cDNA fragment, Se-4 and PLD were homologous with sequence identities of 100%.The expression levels of PLD mRNA in DM group and DM+Se group was higher obviously than that of NC group(P
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Acta Nutrimenta Sinica
Year:
1956
Type:
Article
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