Localization of neural specific transcription factor LMO3 mRNA in adult mouse central nervous system / 第三军医大学学报

ABSTRACT

Objective To explore the localization of neural specific transcription factor LMO3 mRNA in the central nervous system of the mice.Methods Five male adult mice,weighing 60-80 g,were sacrificed,and the brain and spinal cord were fixed and sliced.In situ hybridization histochemistry staining method with digoxigen-lablled cRNA probe was used in detection of LMO3 mRNA.Results The expression of LMO3 mRNA could be observed in cerebrum,cerebellum,thalamus,brain stem and spine.They were localized in somatic and dendritic profiles at most regions of adult mouse central nervous system.The intense hybridization signal could be seen in Ⅱ-Ⅵ layers of cerebrum,piriform cortex,entorhinal cortex,hippocampus CA1 and dentate gyrus,substantia nigra,motor nucleus of trigeminus,nucleus of hypoglossal,red nucleus,paraventricular nucleus,gigantocellular reticular nucleus,lateral reticular nucleus.The moderate staining was detected in olfactory bulb,hippocampal CA2,amygdala nucleus,cingulated anterior cortex,piriform nucleus etc..The faint signal showed in globus pallidus,spinal nucleus of trigeminal,interposed cerebellar nucleus,ambiguous nucleus,lateral cerebellar nucleus,Purkinje cell of cerebellum,thalamus,caudate putamen,amygdaloid body,nuclei raphe.Conclusion The wide presence of LMO3 mRNA in adult mice central nervous system suggests a role of regulation for this gene in learning,memory and olfaction besides in dopamine neurotransmitter regulation.