The Expression of TNF-? mRNA、IL-6mRNA in Liver of Rats with Alcohol Fatty Liver / 医学研究杂志

ABSTRACT

Objective To measure the the expression of TNF-?、IL-6mRNA in liver of rats with alcohol fatty liver, and the amounts of TNF-?and IL-6, to observe the lesion of lipid superoxidation, to explore the pathogenesis of alcohol fatty liver. Methods 23 of SD rats were divided into the normal group(10) and the model group(13),all the rats in the model were given 50% alcohol by daily gavage, and drank 10% alcohol, after 14 weeks, all the rats were killed and the pathological change of liver were observed, the amounts of serum TG,TC,ALT,AST,FFA,MDA,SOD,GSH-PX,TNF-? and IL-6 were examined, the expression of TNF-?、IL-6mRNA was measured. Results In the model ,to different degree, there were some fatty steatosis , the amounts of TG、FFA、MDA、TNF-?and IL-6 were higher than that in the normal group, but the amounts of SOD and GSH-PX lower, in comparison with the normal, the expression of TNF-?mRNAand IL-6mRNA in liver of model were significantly increased. Conclusions long-term of ingestion of potation maybe cause the disorder of lipid metabolic system and alcohol fatty liver came into being. Simultaneously with the enhancement of oxidation stress/lipid superoxidation and abnormity of secretion of some cytokine such as TNF-?and IL-6.