Genetype identification and histologic analysis of PS1/APP double transgenic Alzheimer disease mouse model / 第三军医大学学报

ABSTRACT

Objective To identify the genetype of the PS1/APP double transgenic mouse model,analyse the tissue histology and detect the formation of the senile plaque.Methods The specific primer was designed and the APP gene was amplified by PCR from the mouse genome DNA.The A? deposits,activation of microglia and astrocyte were observed by the congo red staining and immunohistologic methods.Results As compared to the negative control,the PS1/APP mouse displayed A? deposits restricted to cortical and hippocampal structures,the activated microglia and astrocyte surrounded the plaque,thus the classic senile plaque stucture was formed.Conclusion The double transgenic PS1/APP mouse produced by the two mice we bought from American Jackson Laboratory could simulate the specific pathogenesis of Alzheimer disease and be the efficient experimental animal model.