Experimental study on transplantation of Bcl-2 gene transfected islet cells to diabetic rats / 解放军医学杂志

ABSTRACT

Objective To investigate the effect of transplantation of islet allograft with Bcl-2 gene transfection on diabetic rats. Methods Among 26 Wistar rats, 22 were built as model of diabetes, and were divided in to 3 groups. Islet cells expressing Bcl-2 by adenovirus-mediated Bcl-2 gene transfection were transplanted through the portal vein of streptozotocin-induced diabetic rats. The function and rejection of the transplanted islet allograft were evaluated by analysis of blood glucose level and histological examination after transplantation. Results 48h after transplantation, the level of blood glucose was significantly decreased in both transfected and untransfected islets transplantation groups. The euglycemia period of transfected islet cells transplantation group was longer than that of the untransfected islet cells transplantation group (16.4?4.3d vs 6.8?2.2d, P