Cell attachment and proliferation of bone marrow-derived osteoblast on zirconia of various surface treatment
The Journal of Advanced Prosthodontics
;
: 96-102, 2014.
Article
in English
| WPRIM
| ID: wpr-55983
ABSTRACT
PURPOSE:
This study was performed to characterize the effects of zirconia coated with calcium phosphate and hydroxyapatite compared to smooth zirconia after bone marrow-derived osteoblast culture. MATERIALS ANDMETHODS:
Bone marrow-derived osteoblasts were cultured on (1) smooth zirconia, (2) zirconia coated with calcium phosphate (CaP), and (3) zirconia coated with hydroxyapatite (HA). The tetrazolium-based colorimetric assay (MTT test) was used for cell proliferation evaluation. Scanning electron microscopy (SEM) and alkaline phosphatase (ALP) activity was measured to evaluate the cellular morphology and differentiation rate. X-ray photoelectron spectroscopy (XPS) was employed for the analysis of surface chemistry. The genetic expression of the osteoblasts and dissolution behavior of the coatings were observed. Assessment of the significance level of the differences between the groups was done with analysis of variance (ANOVA).RESULTS:
From the MTT assay, no significant difference between smooth and surface coated zirconia was found (P>.05). From the SEM image, cells on all three groups of discs were sporadically triangular or spread out in shape with formation of filopodia. From the ALP activity assay, the optical density of osteoblasts on smooth zirconia discs was higher than that on surface treated zirconia discs (P>.05). Most of the genes related to cell adhesion showed similar expression level between smooth and surface treated zirconia. The dissolution rate was higher with CaP than HA coating.CONCLUSION:
The attachment and growth behavior of bone-marrow-derived osteoblasts cultured on smooth surface coated zirconia showed comparable results. However, the HA coating showed more time-dependent stability compared to the CaP coating.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Osteoblasts
/
Pseudopodia
/
Microscopy, Electron, Scanning
/
Cell Adhesion
/
Chemistry
/
Calcium
/
Durapatite
/
Cell Proliferation
/
Alkaline Phosphatase
/
Photoelectron Spectroscopy
Language:
English
Journal:
The Journal of Advanced Prosthodontics
Year:
2014
Type:
Article
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