Effects of compound hyperosmotic solution on ionic pump functions of cardiomyocyte cell membrane of rat after resuscitation from hemorrhagic shock / 解放军医学杂志

ABSTRACT

Objective To explore the changes in ionic pump functions of cardiomyocyte cell membrane and the effects of compound hyperosmotic saline solution. Method Ninety-six Wistar rats were randomized into two groups hyperosmotic saline solution (HES, n=48) and 0.9% sodium chloride solution injection (NS, n=48). Hemorrhagic shock model was reproduced by a gradient volume-control phase exsanguination, combined with an adjustment phase, from femoral artery, with mean arterial pressure kept at 40kPa. At each time-point (pre-shock stage, shock, and 30min, 60min, 90min, 120min post-resuscitation), eight rats were used for various tests. The ATPase activity of cardiomyocyte cell membrane and the myocardial pathologic changes were investigated at different time points. Results Compared with shock stage, the ATPase activity of group HSH was significantly different (P