NS398 on cell apoptosis in neonatal rat brain after hypoxic-ischemic brain damage / 第三军医大学学报

ABSTRACT

Objective To study the cell apoptosis after injecting NS398 in neonatal rat brain injured by hypoxia and ischemia.Methods The model of hypoxic-ischemic brain damage(HIBD)was established in 48 Wistar rats.Intraperitoneal injection of NS398 at dose of 5,20,40 mg/kg respectively was carried out in 36 HIBD rats 30 min before hypoxia.There were also sham operation group in which the normal saline took the place of NS398.Ischemic cortex and hippocampus were sampled for analysis at 24 h,and 7 d after the onset of hypoxic-ischemic damage.The expression and number of apoptotic cells in the cortex and hippocampus were examined with TUNEL.Results The percentage of TUNEL-positive cells in HIBD group was higher than that of sham operation group.The percentage of TUNEL-positive cells in NS398 groups significantly decreased as compared with that of HIBD group.NS398 group at dose of 20 mg/kg was of no difference with NS398 group at dose of 40 mg/kg,but significantly lower than NS398 group at dose 5 mg/kg.Conclusion NS398 can reduce the TUNEL-positive cells,and 20 mg/kg and 40 mg/kg are the more effective dosage.NS398 is of the effective capability to inhibit the brain damage in the growth period when HI happened.