HBV-specific CTL responses in HLA-A2 transgenic mice induced by lipidated Th/CTL constructs / 第三军医大学学报

ABSTRACT

Objective To explore how to trigger in vivo HBV-specific HLAⅠ-restricted CTL responses to exogenously synthesized polypeptides.Methods The polypeptide was designed and synthesized based on a common helper T lymphocyte(Th) sequence of tetanus toxoid,the immunodominant CTL epitope of HBcAg and B-epitope of PreS2.The polypeptide was given to the transgenic mice respectively after being emulsified in complete or incomplete Freund's adjuvant(CFA/IFA),or inducted to 2 palmitic acid molecules(palm2-KSS,as built-in adjuvant) at its amino termini.The immunological properties of mimicing human CTL responses to HBV proteins were studied in these immunized mice.Results The HTL-CTL epitope construct with lipidated built-in adjuvant could trigger specifically and effectively vigorous in HLA-A2 transgenic mice,and dramatic difference was found compared with the mimic polypeptides emulsified in CFA and IFA(P