EFFECTS OF GRAPE JUICE ON PROLIFERATION AND APOPTOSIS OF HUMAN PROSTATE CARCINOMA PC-3 CELLS / 营养学报
Acta Nutrimenta Sinica
;
(6)2004.
Article
in Chinese
| WPRIM
| ID: wpr-560258
ABSTRACT
Objectives:
To study the inhibitory effects of grape juice on human prostate carcinoma PC-3 cells and its possible mechanism.Method:
PC-3 cells were treated with grape juice in different concentration(16, 32, 64 ?l/ml) and resveratrol for 48 h; and the proliferation of PC -3 cells were measured by growth curve and MTT assay. TUNEL was used to observe the morphology of apoptotic cells and flow cytometry to analyze the PC-3 apoptosis.Results:
Resveratrol and grape juice could markedly inhibit the proliferation of PC-3 cells, and TUNEL positive cells were detectable. The percentages of apoptotic cells were 6.0%,18.5%,30.0%, 12.7% in experimental groups and resveratrol group respectively. The effect on PC-3 cells was enhanced with increasing amount of grape juice. The effect of low dose group was weaker than that of resveratrol group, but medium and high dose groups were stronger.Conclusion:
The anti-prostate cancer effect of grape juice is not only related to resveratrol contained but also to synergism with other components, and its mechanism might be the induction of PC-3 cells apoptosis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Acta Nutrimenta Sinica
Year:
2004
Type:
Article
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