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Study on variations of mtDNA from high and low metastatic mouse hepatocarcinoma cell sublines / 第三军医大学学报
Article in Zh | WPRIM | ID: wpr-560280
Responsible library: WPRO
ABSTRACT
Objective To investigate the variations of mtDNA from high and low metastatic mouse hepatocarcinoma cell sublines Hca-F and Hca-P, and the relationship between mutations of mtDNA and carcinogenesis. Methods The variations of D-loop, ND3 and tRNA Met+Glu+Ile gene fragments of mtDNA from Hca-F and Hca-P cells were analyzed by PCR-RFLP and sequencing techniques. Results No amplification fragment length polymorphism and restriction fragment length polymorphism were observed in tRNA Met+Glu+Ile , ND3 and D-loop of mtDNA from the 2 cell sublines. Sequence difference between these 2 cell sublines were found in mtDNA D-loop region by sequencing. Conclusions Genetic alteration of mtDNA non-coding region in tumors, which may reflect the environmental and genetic influences operative during tumor progression, can be linked to their tumorigenic phenotype.
Key words
Full text: 1 Index: WPRIM Language: Zh Journal: Journal of Third Military Medical University Year: 2003 Type: Article
Full text: 1 Index: WPRIM Language: Zh Journal: Journal of Third Military Medical University Year: 2003 Type: Article