Influence of granulocyte-colony stimulating factor combined with adrenocortical steroid and/or immunosuppressants on immune system of donor mice during hematopoietic stem cell mobilization / 第二军医大学学报

ABSTRACT

Objective:To explore the influence of granulocyte-colony stimulating factor(G-CSF) combined with adrenocortical steroid and/or immunosuppressants on immune system of donor mice during hematopoietic stem cell mobilization.Methods: The donor C57BL/6 mice were divided into 4 groups according to the mobilization strategies,namely,the G-CSF group((250 ?g/kg) for 4 days),G-CSF(250 ?g/kg for 4 days) + methylprednisolone group(100 mg/kg on day 3),G-CSF((250 ?g/kg) for 4 days) + methylprednisolone group(100 mg/kg on day 3) + cyclosporine A(100 mg/kg on day 3),and PBS control group.The post-mobilization T lymphocytes of C57BL/6 mice and the splenocytes of BALB/c mice were subjected to mixed lymphocyte reactions(MLR).The levels of IFN-?,IL-4,and TGF-?_(1) were determined in the supernatants of donor mice splenocytes by ELISA method and the mRNA levels of IFN-? and IL-4 were determined by RT-PCR.Results: The results showed that the MLR-inhibitory activities in the 3(G-CSF) mobilized groups were 42%,21%, and 39%,respectively;all lower than that in PBS control group(68%).IFN-? levels in the 3 G-CSF mobilized groups were lower than that in PBS control group,but he levels of IL-4 and TGF-?_(1)were significantly higher than those in PBS group(P