Expression of NF-?B P65 in mouse to rat cardiac xenograft model / 第三军医大学学报

ABSTRACT

Objective To reproduce mouse to rat heart transplantation model and determine the expression and DNA binding activity of NF-?B P65 in xenograft heart.Methods Six NIH mice were used as heart donors and six Wistar rats as heart recipients.After the transplantation,the transplanted hearts without any drug intervention were prepared for experiment after cardiac arrest.The hearts from another six NIH mice served as normal controls.The expression of NF-?B P65 protein and NF-?B P65 DNA binding activity in xenograft heart were determined by Western blotting and electrophoretic mobility shift assay(EMSA).Results The expression level of NF-?B P65 and NF-?B P65 DNA binding activity in transplanted hearts were higher than control hearts(P