Role of Caspase-1 activated cytokines in renal injury of experimental severe acute pancreatitis / 解放军医学杂志

ABSTRACT

Objective To study the role of Caspase-1 activated cytokines in renal injury of experimental severe acute pancreatitis(SAP).Methods Thirty-two SD rats were randomly divided into four groupshealthy control(HC),SAP 6h,SAP 12h and SAP 18h groups.SAP was induced by retrograde infusion of 5% sodium taurocholate into the bili-pancreatic duct in SD rats.Serum BUN and Cr were determined by an automated HITACHI-7150 analyzer.Serum IL-1? level was determined by ELISA.Myeloperoxidase(MPO)in the kidney was determined by enzyme chemistry assay.Intrarenal expressions of Caspase-1,IL-1? and IL-18 mRNA were assessed by semi-quantitative RT-PCR.Results The serum BUN and Cr levels were increased significantly(P