Real-time quantification of microRNAs by RNA-tailing and primer-extension RT-PCR

Qi ZHANG; Xiangjun HE; Xiuying PAN

Real-time quantification of microRNAs by RNA-tailing and primer-extension RT-PCR / 北京大学学报(医学版)

Qi ZHANG; Xiangjun HE; Xiuying PAN.

Journal of Peking University(Health Sciences) ; (6)2004.

Article in Chinese | WPRIM | ID: wpr-560942

ABSTRACT

ABSTRACT

Objective:

To optimize and evaluate the modified RNA-tailing and primer-extension RT-PCR method in relative quantification of microRNAs (miRNAs) in several kinds of tissues.

Methods:

Small-sized RNAs (

MicroRNAs; Reverse transcriptase polymerase chain reaction; Laboratory techniques and procedures

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Journal of Peking University(Health Sciences) Year: 2004 Type: Article