THE PROTECTIVE EFFECTS OF FOLIC ACID,VB_6,VB_(12) TO RAT AORTIC ENDOTHELIAL CELLS INJURIED BY DL-HOMOCYSTEINE / 营养学报

ABSTRACT

Objective:To investigate the cross protective effects of folic acid,VB6,VB12 on rat aortic endothelial cells(RAEC) from injury induced by DL-homocysteine(Hcy).Method:RAEC were cultured in vitro,and the cross effects of folic acid,VB6,VB12 were studied by MTT.According to the results of orthogonal design,the activities of SOD,GSH-PX,NOS and the contents of MDA,NO in the media were compared.Results:The results of orthogonal design indicated that the inhibition of Hcy to RAEC could be relieved by folic acid,VB12 excluding VB6.The interaction existed between folic acid and VB12,as well as VB6 and VB12.The inhibition of Hcy to the enzymes(SOD、GSH-PX、NOS) could be relieved by three vitamin projects.Folic acid,VB12,VB6 could inhibit the reduction of NO and decrease the production of MDA.Conclusion:The simultaneous addition of folic acid,VB6 and VB12 may be an excellent strategy for protection of RAEC injury induced by DL-homocysteine.