Photocytotoxicity characteristics of two photosensitizers induced by copper vapor laser irradiation / 解放军医学杂志

ABSTRACT

Objective To investigate the 50% inhibition concentration(IC50)of haematoporphyrin derivative(HpD)and hematoporphyrin monomethyl ether(HMME)at the saturated light dose and 50% inhibition energy density(IED50)at saturated photosensitizer dose in order to find out their photocytotoxicity features.Methods Cell culture and MTT technology were utilized to inspect photosensitizers' photocytotoxicity to the endothelial cells of mouse lung vessel.Exponentially growing cells were incubated with varying concentrations of the photosensitizers in DMEM medium(low glucose)in 96-well microliter plate for 4h.The plate was then exposed to copper vapor laser at 510.6nm and 578.2nm wavelength,respectively,for a period of time.Incubation was continued for another 24h in darkness,and the number of surviving cell was analyzed by MTT assay.Results The findings suggested that the IC50 of HMME was 1.31 and 1.24 times of that of HpD in 510.6nm and 578.2nm wavelengths at saturated light dose,respectively.IED50 of HPD was 1.18 and 1.17 times of that of HMME in 510.6nm and 578.2nm wavelengths at saturated photosensitizer dose,respectively.Conclusion HpD has stronger photocytotoxicity than HMME under 510.6nm and 578.2nm wavelength exposure,and the photocytotoxicity is closely related to light dose and wavelength.