The Application Study of Telomerase and P53 Gene in Bronchoalveolar Lavage Fluids in Patients with Lung Cancer / 医学研究杂志

ABSTRACT

Objective To investigate the diagnostic value of telomerase activity and mutatioms of P53 gene in bronchoalveolar lavage fluids (BALF) in lung cancer. Methods Collect BALF from 86 patients with lung cancer and 82 patients with benign bronchial-pulmonar diseases. Telomeric Repeat Amplificaton Protocol (TRAP) and Polymerase Chain Reaction Single Strand Conformational Polymorphism(PCR-SSCP) were used in this study. Meanwhile, conduct fibrobronchoscope brush biopsy and protractor biopsy. Results ①The positive rate of telomerase in experimental group is 82.56% and that of mutatioms of P53 gene is 32.56%, which are both higher than 4.48% and 0% (P0.05) between the mutatioms of P53 and the expression of telomerase activity with respect to pathological classification of lung carcinomas. ③ In central-type lung cancer group, the combining positive rate of telomerase and mutatioms of P53 is 94.34%, the positive rate of routine fibrobronchoscope practice is 86.79%,there were no statistical differences between them. In peripheral lung cancer group, the combining positive rate of telomerase and mutatioms of P53 is 75.76%, which is higher than the positive rate of routine fibrobronchoscope practice that is 45.45%(P