A simplified method for rapid isolation of Schistosoma japonicum immature eggs / 中国血吸虫病防治杂志

ABSTRACT

Objective To study a simplified method for rapid isolation of the Schistosoma japonicum immature eggs for resolving the problems of the routine method which is tedious and difficult to get intact and high-purity eggs. Methods A modified method was devised on the basis of the previous experience through repetitive sievings followed by trypsin digestion and centrifugations. Results The immature eggs with fairly high purity were obtained within a short time. The isolated eggs remained intact and the rate of recovery was significantly increased. Conclusion This method has paved the way for future molecular biology research of the immature eggs of Schistosoma japonicum.