Changes of reactive oxygen species and mitochondrial membrane potential in mitochondrial DNA-depleted SK-Hep1 cells / 第三军医大学学报

ABSTRACT

Objective To observe the differences of the reactive oxygen species(ROS) and mitochondrial membrane potential(??m) in mitochondrial DNA-depleted SK-Hep1 and its parent cells.Methods ?0SK-Hep1 cell line was generated by treating SK-Hep1 with ethidium bromide.Flow cytometry(FCM) and laser scanning confocal microscopy were used to detect ROS and ??m.Results Fluorescence intensity of ROS in ?0SK-Hep1 cells was significantly higher than that of the parent cells(35.5 vs 15.6),and the ??m was lower in ?0SK-Hep1 cells(55.0 vs 65.9).Conclusion ?0SK-Hep1 cells showed elevated ROS and lower ??m.These changes might contribute to the variation of phenotypes.