Prokaryotic expression and identification of human HT036 protein / 第三军医大学学报
Journal of Third Military Medical University
; (24)2003.
Article
in Zh
| WPRIM
| ID: wpr-561474
Responsible library:
WPRO
ABSTRACT
Objective To express human HT036 protein in Escherichia coli(E.coli.)and identify it.Methods The cDNA sequence obtained by PCR was cloned into the prokaryotic expression plasmid pET30a(+).The target protein was expressed in E.coli..induced by IPTG and analyzed by Western blotting.Results The interest gene was identified by restriction endonucleases digestion and DNA sequencing.The protein was highly expressed in E.coli..Conclusion We successfully expressed the HT036 protein.
Full text:
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Index:
WPRIM
Type of study:
Diagnostic_studies
Language:
Zh
Journal:
Journal of Third Military Medical University
Year:
2003
Type:
Article