Effect of chronic low frequency electrical stimulation and chronic physiological frequency electrical stimulation on diaphragmatic muscle myosin heavy chain and enzyme activity in emphysema rabbits / 第三军医大学学报

ABSTRACT

Objective To investigate the effect of chronic low-frequency electrical stimulation on the structural and metabolic changes of diaphragm of emphysema rabbits.Methods The rabbit model of emphysema was made by papain-inhaling of ultrasonic atomization once a week for 3 weeks in 30 adult Japanese rabbits,6 receiving 10 Hz chronic low frequency electrical stimulation,6 receiving 40 Hz,and 6 receiving(10+40)Hz.Other 6 emphysema rabbits and 6 normal rabbits served as control.An enzyme chemical method was used to observe the changes in the enzyme activities of sarcoplasmic reticulum Ca2+-ATPase,succinate dehydrogenase(SDH)and the lactate dehydrogenase(LDH)and changes in MHC in control group,emphysema group,and emphysema+chronic low frequency electrical stimulation group.Results Following chronic electrical stimulation using 40 Hz,10 Hz,and(10+40)Hz,the relative contents of MHC were increased in the diaphragmatic muscle of rabbits with emphysema.The percentage of MHC-Ⅰ was significantly increased in the 10 Hz group,while that of MHC-Ⅱa was significantly increased in the(10+40)Hz group.Following chronic electrical stimulation using 40 Hz and(10+40)Hz,diaphragmatic muscle SR Ca2+-ATPase activity was increased in rabbits with emphysema(P