Influence of ERK1/2 signal-transduction pathway on differentiation of mesencephalic neural stem cells induced by bone marrow stromal cell-conditioned medium / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12)1985.
Article
in Chinese
| WPRIM
| ID: wpr-561679
ABSTRACT
Objective:
To investigate the effect of the ERK1/2 signal-transduction pathway on differentiation of rat mesencephalic neural stem cells(NSCs)in presence of bone marrow stromal cell-conditioned medium(BMSCs-CM)in vitro.Methods:
NSCs were cultured on poly-L-lysine(PLL)coated 35 mm dishes.The neurospheres adhered to the bottom 30 min later;then the culture medium of natural differentiation group was replaced by neurobasal medium containing 2% B27;the control group was replaced with BMSCs- CM;and the inhibition group was replaced with BMSCs-CM containing PD98059(5?mol/L).The proportions of neurons and astrocytes and their morphological difference were observed by immunocytochemistry technique 7 d later.Results:
We successfully cultured NSCs from adult rat BMSCs-CM.The proportion of neurons in the natural differentiation group(15.70?10.3)% was lower than that in the control group([51.17?10.30]%,P
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Academic Journal of Second Military Medical University
Year:
1985
Type:
Article
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