Contraceptive effect of fusogenic trophoblast surface epitopes / 第三军医大学学报

ABSTRACT

Objective To synthesize a multiple antigenic peptide(MAP),observe its humoral immune response and evaluate its antifertility potential.Methods Based on fusogenic epitope mimic peptide and synthetic nonnatural Pan DR T Helper Epitope(PADRE),MAP antigenic peptides were designed and synthesized.After suspending with the equivalent Freund's adjuvant,the antigens were used to immunize the female C57BL/6 mice to evalutate their humoral immune response in animals and efficiency of specific antisera on inhibiting syncytial trophoblast fusion.Results The MAP peptide was successfully synthesized.After purified with HPLC,its purity reached 95%.After immunization,the highest level of specific IgG in mice serum was 11 024,and the corresponding antigen of human trophoblastic cell could be identified in the antisera.In presence of mice antiserum by 110 dilution,forskolin-induced intercellular fusion of BeWo choriocarcinoma cells decreased remarkably(P