Expression of estrogen receptor alpha and beta in meibomian gland tissue of rats / 解放军医学杂志

ABSTRACT

Objective To explore distribution of the estrogen receptor alpha(ER?)and estrogen receptor beta(ER?)in meibomian gland tissue of the normal female rats.Methods 22 SD female rats were killed by dislocation of cervical vertebra,the tarsal plate tissues of the upper eyelids were in paraffin imbedding and made to a series of sections which were then studied by means of immunohistochemical method,and the uterus of rats was used as positive control and PBS as negative control.The serum estradiol level of rats was detected by radioimmunoassay.Results ER? and ER? were expressed in acinar epithelia of meibomian glands.Among 22 rats,21 rats(95.4%) were found to be ER? positive expression in the basa cell of the meibomian gland acinar,15 rats(68.2%) were found to be ER? positive expression in transitional cell and mature cell of the meibomian gland acinar.14 rats(63.6%) were found to be ER? positive expression in the basa cell of the meibomian gland acinar,8 rats(36.4%) were found to be ER? positive expression in transitional cell and mature cell of the meibomian gland acinar.The positive staining rate for ER? was higher than that for ER?,which have statistically significant difference(P