The Culture of Rabbit Conjunctival Epithelium in Vitro / 医学研究杂志
Journal of Medical Research
;
(12)2006.
Article
in Chinese
| WPRIM
| ID: wpr-561811
ABSTRACT
Objective To study the culture method of conjunctival epithelium in vitro.Methods The rabbit conjunctival epithelium was cultured by tissue inoculation and was explanted in culture dish or on human amnionic membrane for fwo weeks.The cell morphology and growth features were observed.Results The rabbit conjunctival epithelium explanted in culture dish connected each other into membrane at eighth days around,which were monolayer cells was observed.The rabbit conjunctival epithelium explanted on human amnionic membrane connected each other into membrane at sixth day,and multi-layer cells were observed.All cultured cells showed positive in CK13 staining by indirect immunofluorescence staining.Conclusions Tissue inoculation is a good approach of conjunctival epithelium culture,and multi-layer cells can be gained if the tissue be exlpanted on human amnionic membrane.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Medical Research
Year:
2006
Type:
Article
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