EFFECTS OF FOLIC ACID, VITAMIN B_6 AND B_(12) ON PLASMA HOMOCYSTEINE LEVELS AND ANTIOXIDATIVE ABILITIES IN RATS WITH CEREBRAL ISCHEMIA / 营养学报

ABSTRACT

Objective: To investigate the effects of folic acid (FA), vitamin B6 (VB6) and B12 (VB12) on plasma homocysteine (Hcy) and antioxidative activities in focal cerebral ischemia rats. Method: Rats were randomly divided into four groups including sham operation (Sham), middle cerebral artery occlusion model (MCAO), MCAO+FA and MCAO+FA +VB6+VB12(MCAO+CV). MCAO model was induced by operation. Plasma Hcy, serum and brain SOD and GSH-Px activities and MDA content in rats were measured before and 28 d after supplementation and 24 h after ischemia or only after ischemia. Results: Plasma Hcy in MCAO+FA and MCAO+CV group were significantly lower than those in Sham and MCAO groups after supplementation and ischemia, and the MCAO+CV group lower than MCAO+FA group. Serum and brain SOD and GSH-Px activities were significantly higher, and MDA contents lower in MCAO+FA and MCAO+CV groups than those in MCAO group. Conclusion: Supplementing FA, VB6 and VB12 can reduce plasma Hcy, improve antioxidative abilities and decrease the injury by oxidative stress following cerebral ischemia.