Effects of indomethacin on inflammatory process induced by focal cerebral ischemia-reperfusion in rats and its mechanism / 解放军医学杂志

ABSTRACT

Objective Cerebral ischemia would rapidly initiate structural and functional damages in brain, including blood-brain barrier disruption, inflammation, and angiogenesis. The purpose of present study is to investigate the effects of indomethacin, an agent that inhibits cyclooxygenase, on the inflammatory reaction after cerebral ischemia-reperfusion in transient middle cerebral artery occlusion(MCAO)rats and its possible mechanism. Methods Adult male SD rats weighed 250-250g were subjected to either sham surgery or middle cerebral artery occlusion (MCAO) for 2h of brain ischemia and 24h reperfusion. After 24h of reperfusion,the size of cerebral infarction and the neurological deficit were determined by the method of TTC (2,3,5-Triphenyltetrazolium Chloride) staining and Longa's score analysis.The contents of IL-8, IL-1?, TNF-? and MPO in brain tissue were assayed by ELISA. The expressions of ICAM-1 ande E-selectin were evaluated with Western-blot. Results It was observed that indomethacin (6 or 9mg/kg i.p pretreatment for 5d, once a day) significantly reduced cerebral infarct volume and ameliorated the neurological deficit (P