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Role of MHC class Ⅱ transactivator in upregulating the expression of HLA class Ⅱ antigen in HepG2 cells / 第三军医大学学报
Article in Zh | WPRIM | ID: wpr-562469
Responsible library: WPRO
ABSTRACT
Objective To investigate the role of MHC class Ⅱ transactivator(CⅡTA) in upregulating the expression of HLA class Ⅱ antigen in HepG2 cells.Methods Eukaryotic expression vector EBS-NPL-CⅡTA containing CⅡTA cDNA or the empty vector EBS-NPL was transfected into HepG2 cells respectively.CⅡTA mRNA and HLA class Ⅱ antigen(HLA-DR,DP,DQ) were respectively detecued by RT-PCR,indirect cell immunofluorescence technique and flow cytometry in original HepG2 cells,HepG2 cells transfected with EBS-NPL-CⅡTA or the empty vector EBS-NPL.Results The expression of CⅡTA mRNA and HLA class Ⅱ antigen(HLA-DR,DP,DQ) were not observed in original HepG2 cells and HepG2 cells transfected with empty vector,but in the HepG2 cells transfected with EBS-NPL-CⅡTA.Conclusion CⅡTA is a switching factor of mastering the expression of HLA class Ⅱ antigen in HepG2 cells.The lack of CⅡTA expression in HepG2 cells contributes to no expression of HLAⅡ antigen.
Key words
Full text: 1 Index: WPRIM Language: Zh Journal: Journal of Third Military Medical University Year: 2003 Type: Article
Full text: 1 Index: WPRIM Language: Zh Journal: Journal of Third Military Medical University Year: 2003 Type: Article