Melatonin inhibits the regulation of hypothalamo-pituitary-adrenal axis in diabetic rats / 第二军医大学学报

ABSTRACT

Objective:To study the effect of melatonin(Mel),a potent antioxidant,on the oxidative stress and the activity of hypothalamo-pituitary-adrenal axis in diabetic rats.Methods:Male SD rats were assigned to the following 4 groupsnormal control(treated with 2% ethyl alcohol lavage\[NC,n=6\]),diabetes mellitus control(treated with 2% ethyl alcohol lavage \[DM,n=7\]),diabetes mellitus model group 1(treated with Mel lavage \[10 mg?kg-1?d-1,DM+Mel1,n=6\]),diabetes mellitus model group 2(treated with Mel lavage \[0.2 mg?kg-1?d-1,DM+Mel2,n=6\]).Diabetes in DM group,DM+Mel1 group and DM+Mel2 group was induced by intraperitoneal injection of streptozotacin(65 mg/kg).Blood glucose,body weight and adrenal gland weight/body weight(AW/BW)were determined in the 4 groups 12 weeks later;malondialdehyde(MDA)was examined with visible spectrophotometry;blood total cholesterol(TC)and triglyceride(TG)were examined with enzymology method;blood corticotrophin-releasing hormone(CRH),adreno-corticotrophic hormone(ACTH)and corticosterone(CORT)were examined with radioimmune method.Results:Blood glucose,MDA,TC,TG,CRH,ACTH,CORT and AW/BW in DM group were significantly higher than those in NC group(P