Effect of leflunomide on NF-?B P65 in mouse to rat cardiac xenograft / 第三军医大学学报
Journal of Third Military Medical University
;
(24)2003.
Article
in Chinese
| WPRIM
| ID: wpr-562555
ABSTRACT
Objective By reproducing mouse to rat heart xenograft,to determine the effect of leflunomide on NF-?B P65 in the transplanted heart.Methods The recipient rats were randomly divided into 4 groupsgroup A(n=6)receiving no immunosuppressant as control;group B(n=6)cyclosporine(CsA);group C(n=6)leflunomide(Lef),group D(n=6)Lef plus CsA.The expressions of NF-?B P65 protein and NF-?B P65 DNA binding activity in transplanted heart tissues were determined by immunohistochemistry,Western blot and electrophoretic mobility shift assay(EMSA).Results Administration of CsA alone had no appreciable effect on the survival time of xenografts [(2.50?1.05)d] and could not suppress the protein expression of NF-?B P65 [(263.09?28.81),IOD] and NF-?B P65 DNA binding activity [(227.49?14.83),IOD] in the tissues of xenografts.Administration of Lef alone could significantly prolong the survival time of xenografts [(4.17?1.33)d] and could suppress the protein expression of NF-?B P65 [(173.48?5.85),IOD] and NF-?B P65 DNA binding activity [(109.96?12.46),IOD](P
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Third Military Medical University
Year:
2003
Type:
Article
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