Effects of carbachol on gastric emptying and gastric blood flow in oral fluid resuscitation of burn shock in rats / 解放军医学杂志

ABSTRACT

Objective To observe the effects of carbachol(a cholinergic agent)on gastric emptying and gastric blood flow in resuscitation of early shock with oral administration of glucose-electrolyte solution(GES)in scalded rats.Methods Seventy-eight male Wistar rats were randomly assigned into five groupsshame scald(n=6),scald without fluid resuscitation(n=18),scald resuscitated with oral GES alone(n=18),scald resuscitated with oral carbachol alone(CAL,n=18),and scald resuscitated with oral carbachol plus GES(GES/CAL,n=18).About 35% TBSA scalded injury on back of rats was produced with boiling water(100℃,15s)in the latter four groups.GES(2ml/kg,which is equivalent to half volume of fluid infusion in first 24 hour according to Parkland' formula)was given twice in two-hour intervals by gavage beginning from 30min after the injury in GES and GES/CAL group.Carbachol(60?g/kg)was gavaged 30min after the injury in CAL and GES/CAL group.The gastric emptying rate was determined by phenol red emptying method and gastric blood flow was measured with laser Doppler at 2h,4h and 6h after the scald injury.Results The gastric emptying was obviously delayed and gastric blood flow was significantly reduced in scald animals compared with those in sham group(P