EFFECT OF PHYTIC ACID ON EXPRESSIONS OF APOPTOSIS ASSOCIATED PROTEIN FAS、BAX AND CASPASE-3 IN SGC-7901 CELLS / 营养学报
Acta Nutrimenta Sinica
;
(6)2004.
Article
in Chinese
| WPRIM
| ID: wpr-562636
ABSTRACT
Objective:
To explore the effect of phytic acid on apoptosis of SGC-7901 cells and its mechanisms.Method:
The inhibiting action of phytic acid on SGC-7901 cells was examed by MTT assay,and the effect on apoptosis of SGC-7901 cells was examined by DNA gel electrophoresis,and the expressions of Fas,Bax and caspase-3 protein were detected by Western blot method.Results:
From MTT assay,we observed that phytic acid inhibited the growth of SGC-7901 cells in dose and time dependent manner. Typical DNA ladder was found during gel eletrophoresis. Caspase-3 can be activated by phytic acid and the expressions of Bax and Fas protein in phytic acid group were higher compared with control group,and increased in a dose-dependent manner.Conclusion:
Phytic acid can induce apoptosis of SGC-7901 cells which may be correlated to apoptosis associated protein Fas,Bax and caspase-3.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Acta Nutrimenta Sinica
Year:
2004
Type:
Article
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