Protective effects of green tea polyphenol(-) epigallocatechin gallate on cultured cortical neurons in mice / 中国药理学通报

ABSTRACT

Aim To observe the protective effects of epigallocatechin gallate(EGCG)on cortical neurons of embryonic mice the injury by FeSO4/H2O2 in vitro.Methods The cell injury model was established with FeSO4/H2O2 for 24 h.The process of the growth and morphology of the neurons were observed under phase contrast microscope in vitro.The cell survival was analyzed using colormetric neutral red assay.The cortical neuronal viability was analyzed using MTT assay.TBARS method was performed to detect the levels of Malondialdehyde(MDA).The composing capacity of total protein was measured by BCA method.Results As compared with FeSO4/H2O2,EGCG enhanced the mouse cortical neuronal survivality and viability,decreased the levels of MDA and increased the composing capacity of total protein.Conclusion EGCG exerted protective effects on cortical neurons the injury induced by FeSO4/H2O2,which may be related to free radial scavenging activity and lipid peroxidation process being inhabited.