Growth-inhibiting effects of paclitaxel combined with survivin-specific siRNA on human lung cancer cell lines / 解放军医学杂志

ABSTRACT

Objective To assess the effects of survivin-specific siRNA on chemosensitivity of lung cancer cell A549 to paclitaxel in vitro.Methods For determining if survivin-specific siRNA could enhance the responsiveness of lung cancer to paclitaxel,human lung adenocarcinoma cell lines A549(p53 wild-type)were divided into four different treatment groupscontrol,survivin siRNA,paclitaxel and survivin siRNA+paclitaxel.The effects on cell proliferation,cell cycle and apoptosis were analyzed by MTT assay and flow cytometry,respectively.The protein expression levels of survivin,p21 and PARP were evaluated by Western blot experiments.Results Survivin-specific siRNA showed a augmenting effect on the chemosensitivity of different concentrations of paclitaxel(P