Effects of combination of octreotide and wortmannin on proliferation of gastric carcinoma cell BGC-823 / 中国实用内科杂志
Chinese Journal of Practical Internal Medicine
;
(12)2001.
Article
in Chinese
| WPRIM
| ID: wpr-563076
ABSTRACT
Objective To investigate the effect and mechanism of octreotide with or without wortmannin on the growth of gastric carcinoma cell BGC-823.Methods From June to August of 2007,in Remin Hospital of Wuhan University,cells were exposed to octreotide for four dilution(10-5、10-4、10-3、10-2g/L)with or without wortmannin(a special inhibitor of PI3'K/Akt pathway,the dilution is 40 nmol/L).And then the cytotoxicity was assessed by determining cell survival with MTT after 24 hours,with also were exposed to octreotide for the dilution of 10-3 g/L with or without Wortmannin in 12 h,24 h,36 h,48 h,then the cell survivral rate was accounted and the results were formulated in a table,and the cell cycle was checked-out with Flow Cytometer(FCM);the expression level of p27 gene and protein was determinated with RT-PCR and Western-bloting Test.Results The five groups within one reagent treating were compared with control group,and the results had difference,but the combination of two reagents group had significant difference(P
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Practical Internal Medicine
Year:
2001
Type:
Article
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