Optimization of Experimental Conditon in the PEG Precipitation Associated with Electrochemiluminescence Test to Detect Macroprolactinaemia / 医学研究杂志
Journal of Medical Research
;
(12)2006.
Article
in Chinese
| WPRIM
| ID: wpr-563179
ABSTRACT
Objective The experimental condition of PEG precipitation pre-treatment method associated with electrochemiluminescence was optimized to gain a coincident result compared with gel chromatography method.Methods A fixed centrifuge time,optimized centrifuge speed,temperature and iron intensity were used to achieve a best experimental condition.Results The detection concentration of micromolecular PRL was approximate to gel chromatography method under the experimental condition of being centrifuged 10 minutes at 18℃ and pretreated with 25%PEG 6000 1M PBS.Conclusions The optimized PEG precipitation test united with the electrochemillumescence immunoassy was a good method for screening macroprolactinemia in clinical diagnosis.
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Medical Research
Year:
2006
Type:
Article
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