Effect of amino acids on inhibition of lactate dehydrogenase-X by gossypol
Experimental & Molecular Medicine
;
: 25-29, 1999.
Article
in English
| WPRIM
| ID: wpr-56322
ABSTRACT
Gossypol acetic acid (GAA) has been shown to have male antifertility effects, but there are pronounced differences among animal species. In the search of endogenous effector molecules, which interfere with the functions of GAA, we have studied the in vitro effect of various amino acids on the inhibition of the purified LDH-X by GAA. Histidine, cysteine and glycine were shown to block the effect of GAA. The effects of these amino acids were concentration dependent. Histidine and glycine protection was found to be complex type in which both the Km and Vmax were decreased compared to control. Arginine, glutamic acid, phenylalanine and valine were found to be ineffective against the inhibitory action of GAA.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Spermatocidal Agents
/
Testis
/
Goats
/
Gossypol
/
Enzyme Inhibitors
/
Amino Acids
/
Isoenzymes
/
L-Lactate Dehydrogenase
/
Animals
Language:
English
Journal:
Experimental & Molecular Medicine
Year:
1999
Type:
Article
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