Effects of propofol on the cytokine release from human monocytes induced by lipopolysaccharide / 解放军医学杂志

ABSTRACT

Objective To observe the effects of propofol on the cytokine release from human monocytic cell line (THP1) induced by lipopolysaccharide (LPS). Methods THP1 cells cultured in vitro, and they were divided into 3 groups 0 ?g/ml LPS group, 1 ?g/ml LPS group and 1?g/ml LPS+50?mol/L propofol group, respectively, and incubated for 12 hours. Then the supernatant was collected from each culture for the determination of the levels of granulocyte/macrophage colony stimulating factor (GM-CSF), interferon-? (IFN-?), tumor necrosis factor-? (TNF-?) and interleukin (IL)-1?, IL-2, IL-4, IL-6, IL-8, IL-10, and IL-12 by using LiquiChip system. Propol was added to the culture fluid of THP1 cells in the final concentrations of 0, 12.5, 25, 50 and 100?mol/L (for the groups B, C, D, E and F, respectively), with 1?g/ml LPS. 12 hours later the supernatants were collected to detect the levels of IL-6, IL-8, TNF-?. Culture fluid without LPS and propofol (group A) was used as control. Results The levels of IL-1?, IL-6, IL-8 and TNF-? were elevated obviously in group L than that in control group (P0.05). In group L+P, levels of IL-6, IL-8 and TNF-? were significantly lower than that in group L (P