Establishment and biological characteristics of human colon carcinoma multidrug-resistant cell line HT29/L-OHP / 第三军医大学学报

ABSTRACT

Objective To establish a drug-resistant human colon cancer cell line to oxaliplatin(L-OHP)based on cell line HT29.Methods A resistant cell line-HT29/L-OHP was established by gradually increasing the dose of L-OHP and intermittent administration.The growth curves,multidrug resistance and resistance index of HT29/L-OHP cell line to anticancer agents were detected by MTT assay.The changes of its biological characteristics were determined by light microscopy,electron microscopy,and flow cytometry.Results HT29/L-OHP cell line was established after 3 months,which had stable resistance to L-OHP and a resistance index of 10.64.HT29/L-OHP cells exhibited cross resistance to many other chemotherapeutic agents.As compared with parental cells,the morphological and chromatosome number of HT29/L-OHP were changed;its doubling time was prolonged;and the number of cells in S phase and G0/G1 phase were decreased while in G2/M phase increased.Conclusion HT29/L-OHP cell line shows the typical multidrug-resistant phenotype.