Extraction and purification of the polysaccharides from Dunaliella salina(PDS) residue / 中国海洋药物

ABSTRACT

Objective To study the extraction and purification of polysaccharides from D.salina residue after extraction of ?-carotene.Methods The crude polysaccharide was extracted by hot basic water,and then was isolated and purified by the DEAE-cellulose and Sepharose-4B.Results After having been purified by DEAE-cellulose chromatography,three components named PDS_(1),PDS_(2),PDS_(3)were obtained.Each component was divided into two components by Sepharose-4B chromatography,then six components were obtained.Conclusion The six components were identified to be homogeneous polysaccharide-protein complexes which were composed of sulfate radical and uronic acid.