Combined effect of dibutyl phthalate and benzo(a) pyrene in rat sertoli cells in vitro / 第三军医大学学报

ABSTRACT

90%.Then the purified sertoli cells were treated with the toxicants at different dose,i.e.DBP(0.1,1,10,100,500 ?g/ml),BaP(0.01,0.1,1,10,50 ?g/ml)and their combination DBP+BaP(0.1+0.01,1+0.1,10+1,100+10,500+50 ?g/ml).The viability of sertoli cells was determined by MTT method and the lactate concentration in the cultured medium was detected.Results As compared with DMSO control,the absorbance values in 100 ?g/ml DBP and DBP+BaP(100+10 ?g/ml)groups were significantly increased(P0.05).Lactate concentration in 100,500 ?g/ml DBP groups,50 ?g/ml BaP group and DBP+BaP(100+10,500+50 ?g/ml)groups were increased(P