Molecular Epidemiologic Study of VacA Genotypes of Helicobacter Pylori in Zhejiang Province / 医学研究杂志

ABSTRACT

Objective To investigate the the distribution of Helicobacter pylori (H. pylori) vacA gentypes and the relationship between the presence of specific genotypes and clinical diseases in Zhejiang of China. Methods 262 Helicobacter pylori strains were colleted from 8 districts of Zhejiang,chromosome DNA was extracted and polymerase chain reaction (PCR) was carried out to determine the polymorphism of vacA and cagA with specific primers.PCR results were analyzed statistically according to their isolated original and clinical outcomes. Results VacA m1b, vacAm2 and vacAm1bm2 were found positive in 27.10%,4.89% and 4.20% of the 262 H.Pylori strains respectively. There was no significant difference in vacA genotypes among different districts of Zhejiang. 26.47%(27/102)of vacA m1b,66.67%(68/102) vacAm2 and 2.94%(3/102)vacAm1bm2 Helicobacter pylori strains were isolated from chronic gastritis; 29.41%(40/136)of vacA m1b,61.76%(84/136)vacAm2 and 3.68%(5/136)vacAm1bm2 Helicobacter pylori strains were isolated from Peptic Ulcer;and 16.67%(4/24)of vacA m1b,75.00%(18/24) vacAm2 and 4.17%(1/24) vacAm1bm2 Helicobacter pylori strains were isolated from gastric cancer; There was no significant difference in vacA genotypes among different clinical disease. Conclusion CagA+ and vacAs1/ m2 are predominant genotypes of H. Pylori in 8 districts of Zhejiang province. However, the relationship between vacA genotypes of H. pylori and the clinical disease can be identified in this study.