Culture and morphological identification of nanobacteria from prostatic calculus / 第三军医大学学报

ABSTRACT

Objective To explore the distribution of the nanobacteria in the prostatic calculus.Methods Calculus specimens were collected from 40 patients of prostatic calculus during operation.After the stones were crushed and rinsed with saline,the liquid was diluted,filtrated and centrifuged,then cultured in strict aseptic condition.The obtained cells were observed using transmission electron microscopy(TEM),detected with alizarin red staining and Gram staining,and identified by indirect immunofluorescence staining with monoclonal antibody 8D10 against nanobacteria.Results ①After 3 to 4 weeks' culture,a white precipitate adhering to the tube bottom was found.②The positive rate of nanobacteria were 65.0% in 40 calculus patients.③Alizarin red staining and Gram staining indicated nanobacteria were distributed in cluster.④Nanobacteria appeared coccoid or coccobacillary-like in shape and ranged from 100 to 500 nm in size.Conclusion Nanobacteria infection exists in calculus patients.