The establishment of insulin resistant model in vitro and preliminary application for screening drugs / 中国药理学通报

ABSTRACT

Aim To establish an in vitro insulin resistant model of HepG2 cells and 3T3-L1 adipocyte and to screen drug in vitro.Methods The insulin resistant models of HepG2 and 3T3-L1 adipocyte were induced by high concentration of insulin and by dexamethasone,respectively.The glucose consumption ofcells was detected after administration with different drugs. Results In the concentration of 10-6 mol?L-1 insulin for 36 hours,HepG2 cells were resistant to insulin and the insulin resistance was maintained for 48 hours without change of cell morphology.After 3T3-L1 adipocyte insulin resistance was induced by dexamethasone,the maximal difference of glucose consumption between the blank control and insulin resistant model group was observed at 96h after dexamethasone administration,but the insulin resistant status had only been maintained for 24 hours without dexamethasone.The glucose consumptions of insulin resistant model of HepG2 and 3T3-L1 adipocyte were promoted by some drugs such as stachyose,berberine and ginsenoside.Conclusions The insulin resistant model of HepG2 cell and 3T3-L1 adipocyte was successfully established in vitro by high concentration of insulin and by dexamethasone,respectively.It can be used to screen drugs for treatment of insulin resistance.