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Development of a multiplex PCR assay to detect Edwardsiella tarda, Streptococcus parauberis, and Streptococcus iniae in olive flounder (Paralichthys olivaceus)
Journal of Veterinary Science ; : 163-166, 2014.
Article in English | WPRIM | ID: wpr-56420
ABSTRACT
A multiplex PCR protocol was established to simultaneously detect major bacterial pathogens in olive flounder (Paralichthys olivaceus) including Edwardsiella (E.) tarda, Streptococcus (S.) parauberis, and S. iniae. The PCR assay was able to detect 0.01 ng of E. tarda, 0.1 ng of S. parauberis, and 1 ng of S. iniae genomic DNA. Furthermore, this technique was found to have high specificity when tested with related bacterial species. This method represents a cheaper, faster, and reliable alternative for identifying major bacterial pathogens in olive flounder, the most important farmed fish in Korea.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Streptococcal Infections / Streptococcus / Flatfishes / Sensitivity and Specificity / Edwardsiella tarda / Enterobacteriaceae Infections / Multiplex Polymerase Chain Reaction / Fish Diseases / Fisheries Type of study: Diagnostic study / Prognostic study Limits: Animals Language: English Journal: Journal of Veterinary Science Year: 2014 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Streptococcal Infections / Streptococcus / Flatfishes / Sensitivity and Specificity / Edwardsiella tarda / Enterobacteriaceae Infections / Multiplex Polymerase Chain Reaction / Fish Diseases / Fisheries Type of study: Diagnostic study / Prognostic study Limits: Animals Language: English Journal: Journal of Veterinary Science Year: 2014 Type: Article