The Study on Content of Myosin Light Chain Phosphatase of SHR' Different Organs / 医学研究杂志

ABSTRACT

Objective To investigate the relationship between MLCP and hypertension pathomechanism of SHR by comparing 130 kD、38 kD、21 kD subunits in aorta,heart,kidney and brain of spontaneously hypertensive rat(SHR) and Wistar Kyoto rat(WKY).Methods The aorta,heart,kidney and brain were obtained from 10 SHRs and 10 WKY rats,and each tissue was freshly removed and weighed and rapidly frozen in liquid nitrogen,homogenized with buffer solution,total protein concentration was rectified according to the methods of Bradford,and SDS-PAGE、Western blots and chemo-luminescence were carried out to determine the content of three subunits,the results were estimated by ABS.t-Test and ANOVA were used to analyzed data.Results The contents of 130kDa,38kDa,21kDa subunits of SHR among the different organs were markedly different,the highest contents of 130kDa was located in the aorta;but the kidney had the highest contents of 38kDa subunit and 21kDa subunit was the highest in the aorta too.Conclusion The difference among the contents of 130kDa subunit、38kDa subunit and 21kDa subunit of SHR and WKY in the different organs implied that abnormity of MLCP' structure and function was associated with hypertension pathomechanism of SHR.