Significance of scavenger receptor A in rat liver tissues during acute obstructive suppurative cholangitis / 第三军医大学学报

ABSTRACT

Objective To investigate the expression of scavenger receptor A(SR-A) in rat liver tissues during acute obstructive suppurative cholangitis(AOSC) and its relations with inflammatory mediators and hepatic injury.Methods Wistar rat models of AOSC were reproduced by ligating choledochus and inside injecting Escherichia coli O111∶B4.The plasma endotoxin concentrations,TNF-? levels and expressions of SR-A protein and mRNA in liver tissues were assayed by limulus test,ELISA,immunohistochemistry and RT-PCR,respectively.Pathological changes of liver tissues were observed under light microscope.Results With the time prolonged,the plasma endotoxin concentrations and the TNF-? levels gradually increased,but the SR-A protein and mRNA expressions decreased in the rats of experimental group,significantly different with those in sham operation group and normal saline group(P